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PD-1 : PD-L1 [Biotinylated] Inhibitor Screening ELISA Kit

For research use only.

IDComponentsSize
EP130-C01High-bind Plate1 plate
EP130-C02Human PD-110 μg
EP130-C03Biotinylated Human PD-L120 μg
EP130-C04Anti-PD-1 Neutralizing Antibody20 μg
EP130-C05Streptavidin-HRP10 μg
EP130-C06Coating Buffer12 mL
EP130-C0710xWashing Buffer 50 mL
EP130-C08Blocking Buffer50 mL
EP130-C09Substrate Solution24 mL
EP130-C10Stop Solution7 mL

背景(Background)

Immune checkpoint pathway is a focal point of today’s cancer research. PD-1 is one of the best characterized checkpoint proteins. The binding between PD-1 and its ligand PD-L1 suppresses T-cell activation and allows cancer cells to escape from body’s immune surveillance. Therefore, the pharmaceutical inhibition of PD-1 or its ligand has been considered a promising strategy by many oncologists.

应用说明(Application)

The inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new PD-1 pathway inhibitors.

It is for research use only.

重构方法(Reconstitution)

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储(Storage)

Unopened kit should be stored at 2°C -8°C upon receiving. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

原理(Assay Principles)

This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new PD-1 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human PD-L1 to immobilized human PD-1 in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated PD-L1 protein, a human PD-1 protein, an anti-PD-1 neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.

Your experiment will include 4 simple steps:

a) Coat the plate with human PD-1.

b) Add your molecule of interest to the tests.

c) Add human PD-L1-Biotin to bind the coated human PD-1.

d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

Finally, the half maximal inhibitory concentration (IC50) of your compound to PD-1: PD-L1 binding will be determined by comparing OD readings among different experimental groups.

 

典型数据-Typical Data Please refer to DS document for the assay protocol.

PD-1 TYPICAL DATA

INHIBITION OF PD-1: PD-L1 [BIOTINYLATED] BINDING BY ANTI-PD-1 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-PD-1 Neutralizing antibody (Catalog # EP130-C04) (1:1 serial dilution, from 5μg/mL to 0.02μg/mL) was added into PD-1: Biotinylated PD-L1 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

 

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021-50850665(上海)
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order.cn@acrobiosystems.com
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PD-1靶点信息
英文全称:Programmed cell death protein 1
中文全称:细胞程序性死亡-1
种类:Homo sapiens
上市药物数量:16详情
临床药物数量:150详情
最高研发阶段:批准上市
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