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 >  Protein>B7-H7 >B77-C82E9

Biotinylated Cynomolgus B7-H7 / HHLA2 Protein, His,Avitag™

分子别名(Synonym)

B7-H7,HHLA2,B7 Homolog 7

表达区间及表达系统(Source)

Biotinylated Cynomolgus B7-H7, His,Avitag (B77-C82E9) is expressed from human 293 cells (HEK293). It contains AA Ile 21 - Asn 345 (Accession # XP_005548285.1).

Predicted N-terminus: Ile 21

Request for sequence

蛋白结构(Molecular Characterization)

B7-H7 Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 39.1 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

B7-H7 SDS-PAGE

Biotinylated Cynomolgus B7-H7, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

B7-H7 ELISA

Immobilized Biotinylated Cynomolgus B7-H7, His,Avitag (Cat. No. B77-C82E9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human CD28H, Fc Tag (Cat. No. CDH-H5251) with a linear range of 0.1-8 ng/mL (QC tested).

Protocol

 

背景(Background)

B7-H7 (HHLA2) is a newly identified B7 family member that regulates human T-cell functions. B7-H7 was previously known as human endogenous retrovirus-H long terminal repeat associating 2 (HHLA2) with unidentified function. Recently, B7-H7 has been identified as a specific ligand for human CD28H. The B7-H7-CD28H pathway strongly promoted CD4+ T-cell proliferation and cytokine production via an AKT-dependent signaling cascade in the presence of TCR signaling, suggesting B7-H7 comprises a new co-stimulatory pathway. The first IgV domain of B7-H7, which presumably binds to a putative receptor, shows the highest homology to other B7 family members.

 

前沿进展

 
 
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B7-H7靶点信息
英文全称:HERV-H LTR-associating protein 2
中文全称:人类内源性逆转录病毒H长末端重复序列结合蛋白2
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床一期
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