膜杰作
Star Staining
组分（Materials Provided）
| ID | Components | Size |
| EP129-C01 | High-bind Plate | 1 plate |
| EP129-C02 | Human IL-7 R alpha & TSLP R | 25 μg |
| EP129-C03 | Anti- TSLP Neutralizing Antibody | 20 μg |
| EP129-C04 | Human TSLP-Biotin | 10 μg |
| EP129-C05 | Streptavidin-HRP | 10 μg |
| EP129-C06 | Coating Buffer | 12 mL |
| EP129-C07 | 10xWashing Buffer | 50 mL |
| EP129-C08 | Blocking Buffer | 50 mL |
| EP129-C09 | Substrate Solution | 12 mL |
| EP129-C10 | Stop Solution | 7 mL |
背景（Background）
Thymic stromal lymphopoietin (TSLP) exerts its biological effects by binding to a high-affinity heteromeric complex composed of thymic stromal lymphopoietin receptor chain and IL-7Rα.The formation of the ternary complex, TSLPR:TSLP: IL-7Rα,initiates signaling in cells co-expressing TSLPR and IL-7Rα.TSLP has been implicated in a variety of allergic diseases (e.g., atopic dermatitis, bronchial asthma, eosinophilic esophagitis). Therefore, inhibition of TSLP interaction with TSLP receptor complex has been considered a promising strategy to blocks thymic stromal lymphopoietin (TSLP) function and prevents the overreactive immune response to allergic,eosinophilic and other types of airway inflammation associated with severe asthma.
应用说明（Application）
This kit is developed for screening for inhibitors of TSLP binding to human IL-7Rα & TSLP R.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new TLSP pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human TLSP to immobilized human IL-7 R alpha & TSLP R in a functional ELISA assay, and employs a simple colorimetric sandwich ELISA platform.
Your experiment will include 4 simple steps:
a) Coat the plate with human IL-7 R alpha & TSLP R.
b) Add your molecule of interest to the tests.
c) Add human TLSP-Biotin to bind the coated human IL-7 R alpha & TSLP R.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit TSLP: IL-7 R alpha & TSLP Rbinding will be determined by comparing OD readings among different experimental groups.
典型数据-Typical Data Please refer to DS document for the assay protocol.
INHIBITION OF TSLP [BIOTINYLATED]: IL-7 R ALPHA & TSLP R BINDING BY ANTI-TSLP NEUTRALIZING ANTIBODY
Serial dilutions of Anti-TSLP Neutralizing antibody (Catalog # EP129-C03) (1:1 serial dilution, from 8 μg/mL to 0.0156 μg/mL (55.317-0.108 nM)) was added into IL-7 R alpha & TSLP R: TSLP-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
INHIBITION OF TSLP [BIOTINYLATED]: IL-7 R ALPHA & TSLP R BINDING BY ANTI- TSLP & IL-13 BISPECIFIC ANTIBODY
Serial dilutions of anti TSLP & IL-13 bispecific antibody (1:1 serial dilution, from 8 μg/mL to 0.0156 μg/mL (55.317-0.108 nM)) was added into IL-7 R alpha & TSLP R: TSLP-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting (Routinely tested).
