膜杰作
Star Staining
组分（Materials Provided）
| ID | Components | Size |
| EP132-C01 | High-bind Plate | 1 plate |
| EP132-C02 | Human IL-4 R alpha | 30 μg |
| EP132-C03 | Human IL-4 | 20 μg |
| EP132-C04 | Human IL-4-Biotin | 10 μg |
| EP132-C05 | Streptavidin-HRP | 10 μg |
| EP132-C06 | Coating Buffer | 12 mL |
| EP132-C07 | 10xWashing Buffer | 50 mL |
| EP132-C08 | Blocking Buffer | 50 mL |
| EP132-C09 | Substrate Solution | 12 mL |
| EP132-C10 | Stop Solution | 7 mL |
产品概述（Product Overview）
Interleukin-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells to Th2 cells). In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine.
应用说明（Application）
This kit is developed for screening for inhibitors of human IL-4Rα binding to human IL-4.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
原理（Assay Principles）
This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new IL-4 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human IL-4 to immobilized human IL-4 R alpha in a functional ELISA assay, and employs a simple colorimetric sandwich ELISA platform.
Your experiment will include 4 simple steps:
a) Coat the plate with human IL-4 R alpha.
b) Add your molecule of interest to the tests.
c) Add human IL-4-Biotin to bind the coated human IL-4 R alpha.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit IL-4: IL-4 R alpha Rbinding will be determined by comparing OD readings among different experimental groups.
典型数据-Typical Data Please refer to DS document for the assay protocol.
INHIBITION OF IL-4 [BIOTINYLATED]: IL-4 R ALPHA BINDING BY Human IL-4
Serial dilutions of Human IL-4(Catalog # EP132-C03) (1:1 serial dilution, from 5 μg/mL to 0.01 μg/mL (295.86-0.58 nM)) was added into IL-4 R alpha: IL-4-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
