分子别名(Synonym)
Coagulation factor X,F10,factor 10,factor X,Stuart Factor,Stuart-Prower factor
表达区间及表达系统(Source)
Cynomolgus Coagulation factor X, His Tag (F10-C52H9) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Lys 488 (Accession # A0A2K5VHK1-1).
Predicted N-terminus: Leu 24
Request for sequence
蛋白结构(Molecular Characterization)
![Coagulation factor X Structure](/images/structure_Images/F10-C52H9-STC2.jpg)
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 15.7 kDa and 30.3 kDa. The protein migrates as 19 kDa,48-53 kDa and 65-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in 25 mM MES,150 mM NaCl,pH6.0 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
![Coagulation factor X SDS-PAGE](images/sds/F10-C52H9.jpg)
Cynomolgus Coagulation factor X, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
![Coagulation factor X SEC-MALS](images/mals/F10-C52H9-M1.jpg)
The purity of Cynomolgus Coagulation factor X, His Tag (Cat. No. F10-C52H9) is more than 90% and the molecular weight of this protein is around 52-70 kDa verified by SEC-MALS.
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背景(Background)
As the only known physiological activator of thrombin, Factor X is a vitamin K-dependent plasma protease that plays a pivotal role in blood coagulation.