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 >  Protein>SHP-2 >SH2-H5248

Human SHP-2 Protein, His Tag

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分子别名(Synonym)

SHP-2

表达区间及表达系统(Source)

Human SHP-2, His Tag (SH2-H5248) is expressed from E. coli cells. It contains AA Thr 2 - Arg 593 (Accession # Q06124-2).

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

SHP-2 Structure

This protein carries a polyhistidine tag at the N-terminus

The protein has a calculated MW of 69.7 kDa. The protein migrates as 65-66 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

SHP-2 SDS-PAGE

Human SHP-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)

Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site. The specific activity is >3 umol/min/mg, measured under the described conditions (QC tested).

Protocol

SHP-2 ENZYME

Serial dilutions of SHP394 (an orally efficacious inhibitor of protein tyrosine phosphatase SHP2) were added into Human SHP-2 Protein, His Tag(SH2-H5248) enzymatic reactions. The half maximal inhibitory concentration (IC50) is 19.36 nM (Routinely tested).

Protocol

 

背景(Background)

SHP-2, a cytoplasmic SH2 domain containing protein tyrosine phosphatase, is involved in the signaling pathways of a variety of growth factors and cytokines. SHP-2 takes key role in transducing signal relay from the cell surface to the nucleus, and is a important intracellular regulator in mediating cell proliferation and differentiation.

 

前沿进展

 
 
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SHP-2靶点信息
英文全称:Src homology phosphotyrosyl phosphatase 2
中文全称:蛋白酪氨酸磷酸酶-2
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:16详情
最高研发阶段:临床三期
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