膜杰作
Star Staining
| ID | Components | Size |
| RAS103-C01 | Pre-coated Glycoprotein E (VZV) Microplate | 1 plate |
| RAS103-C02 | Positive Control | 100 μL |
| RAS103-C03 | Negative Control | 100 μL |
| RAS103-C04 | HRP-Goat anti-Mouse IgG | 50 μL |
| RAS103-C05 | 10 x Washing Buffer | 50 mL |
| RAS103-C06 | Dilution Buffer | 50 mL |
| RAS103-C07 | Substrate Solution | 12 mL |
| RAS103-C08 | Stop Solution | 7 mL |
背景（Background）
Varicella-zoster virus (VZV) the etiologic agent of chickenpox and herpes zoster [HZ], is highly contagious and still endemic worldwide. Glycoprotein E (gE) is one of the known glycoproteins (gB, gC, gE, gH, gI, gK, gI) of VZV that is most abundantly expressed on the surface of virus and infected cells, playing an important role in viral replication and cell-to-cell spread. The strongly immunogenic gE can provide strong IgG signal in body fluid, which makes it ideal to be developed as an antigen for analysis of Immunogenicity in the development of VZV vaccine.Therefore,It's helpful to develop the Mouse Anti-Varicella Zoster Virus Antibody IgG Titer Serologic Assay Kit (gE) to detection the IgG(GE) titer level in mouse serum during the preclinical stage of vaccine development.
应用说明（Application）
This kit is developed for titer detection of Anti-Varicella Zoster Virus Antibody IgG (gE) in mouse serum.
It is for research use only.
存储 & 运输（Storage & Shipping）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-VZV gE antibodies in Mouse serum by Glycoprotein E (VZV). The Kit consists of Pre-coated Glycoprotein E (VZV) Microplate, an Positive Control, an Negative Control, an HRP-Anti-Goat anti mouse IgG secondary antibody.
Your experiment will include 4 simple steps:
a) The samples and Control are diluted by Dilution Buffer. Add your sample to the plate.
b) Add diluted Secondary antibody HRP-Goat anti-Mouse IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
典型数据-Typical Data Please refer to Ds document for the assay protocol.
For determination of antibody titer: the positive sample was diluted with a gradient, and the antibody titer of the sample corresponds to the highest dilution factor that still yields a positive reading.
