膜杰作
Star Staining
分子别名（Synonym）
Spike,S1 protein,Spike glycoprotein Subunit1,S glycoprotein Subunit1,Spike protein S1
表达区间及表达系统（Source）
SARS-CoV-2 Spike S1, His Tag (BA.2.75/Omicron) (S1N-C52Hz) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1 (T19I, LPP24-26del, A27S, G142D, K147E, W152R, F157L, I210V, V213G, G257S, G339H, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, G446S, N460K, S477N, T478K, E484A, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H)). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2.75).
Predicted N-terminus: Val 16
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 76.8 kDa. The protein migrates as 110-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
电泳（SDS-PAGE）
SARS-CoV-2 Spike S1, His Tag (BA.2.75/Omicron) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized SARS-CoV-2 Spike S1, His Tag (BA.2.75/Omicron) (Cat. No. S1N-C52Hz) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG1 (Cat. No. SPD-M265) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized SARS-CoV-2 Spike S1, His Tag (BA.2.75/Omicron) (Cat. No. S1N-C52Hz) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 1-31 ng/mL (Routinely tested).
Protocol
Immobilized SARS-CoV-2 Spike S1, His Tag (BA.2.75/Omicron) (Cat. No. S1N-C52Hz) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) with a linear range of 0.4-25 ng/mL (Routinely tested).
Protocol
- 176XXXXXXX0
- 从技术服务知道了这家公司，我们在多项目上委托了BLI服务，服务体验很好，数据及报告做的很漂亮。后续就从百普赛斯直接购买了蛋白，实验数据稳定，蛋白纯度及实验响应比较出色，供货稳定。成为了我们的关键试剂。
- 2022-9-5
- 156XXXXXXX8
- 我们采购该蛋白用于检测自主开发的抗TROP-2抗体的亲和力，通过OCTET检测其亲和动力学，我们用已经获批的IMMU132进行了测试，其亲和动力学常数为nM级别(1.05nM)。
- 2022-4-6
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背景（Background）
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
