分子别名(Synonym)
Spike,S protein,Spike glycoprotein,S glycoprotein
表达区间及表达系统(Source)
Rhinolophus affinis coronavirus isolate LYRa11 Spike Trimer, His Tag (SPN-R52H3) is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. It contains AA Asp 18 - Pro 1199 (Accession # A0A023PUW9 (R671A, KV972-973PP)).
Predicted N-terminus: Asp 18
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蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 136.2 kDa. The protein migrates as 160-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant.
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重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Rhinolophus affinis coronavirus isolate LYRa11 Spike Trimer, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Rhinolophus affinis coronavirus isolate LYRa11 Spike Trimer, His Tag (Cat. No. SPN-R52H3) is more than 85% and the molecular weight of this protein is around 482-532 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind Rhinolophus affinis coronavirus isolate LYRa11 Spike Trimer, His Tag (Cat. No. SPN-R52H3) with a linear range of 1-39 ng/mL (QC tested).
Protocol
背景(Background)
The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.