膜杰作
Star Staining
分子别名（Synonym）
IL-11,Interleukin-11,AGIF,Oprelvekin,IL11
表达区间及表达系统（Source）
Cynomolgus IL-11 Protein, His Tag (IL1-C5243) is expressed from human 293 cells (HEK293). It contains AA Pro 22 - Leu 199 (Accession # P20808).
Predicted N-terminus: His
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 21.3 kDa. The protein migrates as 22-26 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
电泳（SDS-PAGE）
Cynomolgus IL-11 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human IL-11 R alpha, Fc,Avitag (Cat. No. ILR-H82F5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Cynomolgus IL-11 Protein, His Tag (Cat. No. IL1-C5243) with a linear range of 5-156 ng/mL (QC tested).
Protocol
+添加评论
- 199XXXXXXX6
- 前期尝试过cyno IL11蛋白的表达，亲和力一直很难达到实验标准。为了节省时间成本选择了ACRO的产品，亲和力检测数据完全达标，而且价格也非常优惠，真是太棒了，后期还会回购。
- 2024-12-20
- 150XXXXXXX2
- 使用Cynomolgus IL-11重组蛋白主要用于血清效价检测，蛋白灵敏度高，特异性强，ELISA检测数据背景很干净。在蛋白供应方面一直选择ACRO的产品，质量一直都是NO1.。
- 2024-9-30
- 199XXXXXXX5
- 第一次使用Cynomolgus IL-11重组蛋白，主要用于筛选与其高亲和力结合突变体，蛋白活性稳定，低浓度筛选富集度也很高，节省成本，性价比极高，值得推荐。
- 2024-9-24
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背景（Background）
Interleukin-11 (IL-11) is a pleiotropic cytokine that stimulates megakaryocytopoiesis, resulting in increased production of platelets, as well as activating osteoclasts, inhibiting epithelial cell proliferation and apoptosis, and inhibiting macrophage mediator production. These functions may be particularly important in mediating the hematopoietic, osseous and mucosal protective effects of IL-11. The cytokine also possesses anti-inflammatory activity, and has been proposed as a therapeutic agent in the treatment of chronic inflammatory diseases, such as Crohn's disease and rheumatoid arthritis. Although IL-11 was initially believed to be restricted to mammals, subsequent studies demonstrated it to be expressed in fish. Despite close similarity in gene structure and conservation of key amino acids between fish and mammalian IL-11, they share relatively low overall amino acid identity and may not necessarily be functionally analogous.
