膜杰作
Star Staining
分子别名（Synonym）
IL-11,Interleukin-11,AGIF,Oprelvekin,IL11
表达区间及表达系统（Source）
Mouse IL-11 Protein, Fc Tag (IL1-M5243) is expressed from human 293 cells (HEK293). It contains AA Pro 22 - Leu 199 (Accession # P47873).
Predicted N-terminus: Pro
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 45.6 kDa. The protein migrates as 50-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 25 mM Arginine, PBS, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Mouse IL-11 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse IL-11 Protein, Fc Tag (Cat. No. IL1-M5243) is more than 90% and the molecular weight of this protein is around 80-105 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Mouse IL-11 R alpha, His Tag (Cat. No. ILR-M52H9) at 5 μg/mL (100 μL/well) can bind Mouse IL-11 Protein, Fc Tag (Cat. No. IL1-M5243) with a linear range of 0.2-10 ng/mL (QC tested).
Protocol
Immobilized Mouse IL-11 Protein, Fc Tag (Cat. No. IL1-M5243) at 5 μg/mL (100 μL/well) can bind Anti-IL11,Human,Human IgG1 (K214R, D356E, L358M) with a linear range of 0.6-39 ng/mL (Routinely tested).
Protocol
背景（Background）
Interleukin-11 (IL-11) is a pleiotropic cytokine that stimulates megakaryocytopoiesis, resulting in increased production of platelets, as well as activating osteoclasts, inhibiting epithelial cell proliferation and apoptosis, and inhibiting macrophage mediator production. These functions may be particularly important in mediating the hematopoietic, osseous and mucosal protective effects of IL-11. The cytokine also possesses anti-inflammatory activity, and has been proposed as a therapeutic agent in the treatment of chronic inflammatory diseases, such as Crohn's disease and rheumatoid arthritis. Although IL-11 was initially believed to be restricted to mammals, subsequent studies demonstrated it to be expressed in fish. Despite close similarity in gene structure and conservation of key amino acids between fish and mammalian IL-11, they share relatively low overall amino acid identity and may not necessarily be functionally analogous.
