膜杰作
Star Staining
分子别名（Synonym）
CD3E & CD3D,CD3 delta & CD3 epsilon
表达区间及表达系统（Source）
HRP-Human CD3E & CD3D Heterodimer Protein, His Tag&Tag Free (CDD-HR2W3) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D)).
Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)
Request for sequence
蛋白结构（Molecular Characterization）
HRP-Human CD3E & CD3D Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 16.6 kDa (CD3E) and 13.4 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
活性（Bioactivity）-ELISA
Immobilized Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) at 1 μg/mL (100 μL/well) can bind HRP-Human CD3E & CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-HR2W3) with a linear range of 0.1-8 ng/mL (QC tested).
Protocol
Immobilized UCHT1 at 1 μg/mL (100 μL/well) can bind HRP-Human CD3E & CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-HR2W3) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Immobilized Mouse Anti-Human CD3 (SP34-2) at 1 μg/mL (100 μL/well) can bind HRP-Human CD3E & CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-HR2W3) with a linear range of 0.1-4 ng/mL (Routinely tested).
Protocol
HRP-Human CD3E & CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-HR2W3) is stable at 37℃ for 48 hours, equivalent to store at -70℃ for 55 months without performance reduction (Routinely tested).
Protocol
HRP-Human CD3E & CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-HR2W3) is stable after freezing and thawing three times (Routinely tested).
Protocol
背景（Background）
T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
