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Human Anti-PD-1 Antibody IgG Titer Serologic ELISA Assay Kit

For research use only.
此产品为在研产品。如果您对此产品有兴趣,请直接联系我们。我们会根据您的需求相应地加速我们的研发进程,并为您进行产品预留。

IDComponentsSize
RAB002-C01Pre-coated Human PD-1 Microplate1 plate
RAB002-C02Positive Control100 μL
RAB002-C03Negative Control100 μL
RAB002-C04HRP-Anti-Human IgG 100 μL
RAB002-C0510 x Washing Buffer 50 mL
RAB002-C06Dilution Buffer50 mL
RAB002-C07Substrate Solution12 mL
RAB002-C08Stop Solution7 mL

背景(Background)

Programmed cell death protein 1 (PD-1) is an inhibitory receptor that is expressed on some tumor cells and causes down regulation of the immune system by reducing T-cell activity. Anti-PD-1 monoclonal antibodies block the PD-1 receptor so the T cells are no longer inhibited and therefore activates the immune response against the tumor.

应用说明(Application)

The kit is developed for titer measurement of Anti-PD-1 Antibody IgG in Human serum.

It is for research use only.

存储(Storage)

The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

原理(Assay Principles)

This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-PD-1 antibodies in Human serum. The Kit consists of Pre-coated with Human PD-1 Microplate , an Positive Control, an Negative Control, an HRP-Anti Human IgG secondary antibody.

Your experiment will include 4 simple steps:

a) The samples and Control are diluted by Dilution Buffer. Add your sample to the plate.

b) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

 

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PD-1靶点信息
英文全称:Programmed cell death protein 1
中文全称:细胞程序性死亡-1
种类:Homo sapiens
上市药物数量:16详情
临床药物数量:150详情
最高研发阶段:批准上市
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