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Protein G-coupled Magnetic Beads (recommended for MPCLIA)

热销产品推荐:The Magnetic Stand (Cat.No. MB-01 & Cat.No. MB-02) can be used in conjunction with Beads.

ItemsDetails
Detection MethodChemiluminescence
Product TypeMagnetic Beads (Protein G)
Quantity Size10mg / 100mg
Physical Appearancelyophilized powder mixture
Particle size2.8µm
Beads SurfaceHydrophilic
Amount of Coupled ProteinAbout 400 pmol (9 μg) Protein G/mg Beads
Binding Capacity5-10ug antibody or Human IgG Fc tag proteins/mg beads
Emission WavelengthMeasured relative light units (RLU) at 430 nm

背景(Background)

The Protein G-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly pure form of protein G. The beads can be used to capture the antibodies in Chemiluminescence procedures. The Protein G binds the heavy chain within the Fc region of most immunoglobulins but not bind dog immunoglobulins. The Protein G-coupled Magnetic Beads is easy to capture the most antibodies, and the bounded antibody have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.

应用说明(Application)

The Protein G-coupled Magnetic Beads is used to capture the most antibodies, it can combination with Acridinium ester markers in chemiluminescence technology, The Acridinium ester markers such as Streptavidin-Acridinium ester can capture the biotinylated proteins or molecules, this allows detection of antigen and antibody binding or antibody screening.

*The Isotype control (Cat. No. SMB-B01) is sold separately.

重构方法(Reconstitution)

See Certificate of Analysis (CoA) for detailed instruction.

存储(Storage)

Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C.

Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.

制剂(Formulation)

Please contact us for detailed information.

Contact us for customized product form or formulation.

 

活性(Bioactivity)-MPCLIA

Protein G MPCLIA

Immobilized 0.04 μg /Test of Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) to the Streptavidin-Acridine ester (Cat. No. STN-NA114, 0.008 μg /Test), incubated with 100 μL/Test of Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at increasing concentration coupled to Protein G-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A002) (10 μg beads/Test). Detection was performed with sensitivity of 3.9 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).

Protocol

Protein G MPCLIA

The MPCLIA assay shows that Protein G-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A002) is stable at 37°C for 10 days.

Protein G MPCLIA

The MPCLIA assay shows that Protein G-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A002) is stable after freezing and thawing 3 times.

Protein G MPCLIA

The MPCLIA assay shows that Protein G-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A002) is stable at 4°C for 30 days.

 

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