膜杰作
Star Staining
分子别名（Synonym）
GDNF,ATF
表达区间及表达系统（Source）
Mouse GDNF, His Tag (GDF-M5245) is expressed from human 293 cells (HEK293). It contains AA Ser 78 - Ile 211 (Accession # P48540).
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蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 17.0 kDa. The protein migrates as 16 kDa, 18 kDa and 22-24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Mouse GDNF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Mouse GDNF, His Tag (Cat. No. GDF-M5245) is more than 90% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.
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背景（Background）
Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta (TGF-beta) superfamily. It potently promotes the survival of of peripheral and central neurons. It signals through GFRα receptors, particularly GFRα1. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.
