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 >  Protein>Midkine >MIE-H5117

Human Midkine / Mdk Protein, premium grade

分子别名(Synonym)

MDK,MEK,Midkine,MK1,MKARAP,NEGF2,Retinoic acid-induced differentiation factor

表达区间及表达系统(Source)

Human Midkine Protein, premium grade (MIE-H5117) is expressed from E. coli cells. It contains AA Val 21 - Asp 143 (Accession # P21741-1).

Predicted N-terminus: Met 1

It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

Request for sequence

蛋白结构(Molecular Characterization)

Midkine Structure

This protein carries no "tag".

The protein has a calculated MW of 13.6 kDa. The protein migrates as 16-17 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

无菌(Sterility)

Negative

支原体(Mycoplasma)

Negative.

纯度(Purity)

>90% as determined by SDS-PAGE.

>95% as determined by SEC-HPLC.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Midkine SDS-PAGE

Human Midkine Protein, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-HPLC

Midkine SEC-HPLC

The purity of Human Midkine Protein, premium grade (Cat. No. MIE-H5117) was greater than 95% as determined by SEC-HPLC.

 

活性(Bioactivity)-ELISA

Midkine ELISA

Immobilized Human Midkine Protein, premium grade (Cat. No. MIE-H5117) at 2 μg/mL (100 μL/well) can bind Human Glypican 2, His Tag (Cat. No. GP2-H52H3) with a linear range of 1-63 ng/mL (QC tested).

Protocol

Midkine ELISA

Immobilized Human Midkine Protein, premium grade (Cat. No. MIE-H5117) at 2 μg/mL (100 μL/well) can bind Human LRP-6 (20-630), Mouse IgG2a Fc Tag (Cat. No. LR6-H5253) with a linear range of 1-31 ng/mL (Routinely tested).

Protocol

 

背景(Background)

Midkine (MK or MDK), also known as neurite growth-promoting factor 2 (NEGF2), is a protein that in humans is encoded by the MDK gene. Midkine is a basic heparin-binding growth factor of low molecular weight, and forms a family with pleiotrophin (NEGF1, 46% homologous with MK). It is a nonglycosylated protein, composed of two domains held by disulfide bridges. It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair.

 

前沿进展

 
 
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Midkine靶点信息
英文全称:Midkine
中文全称:中期因子
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床阶段不明
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