膜杰作
Star Staining
优势特色（Features）
With two NLS, which leads to transport to the nucleus efficiently.
High cleavage activity
产品描述（Product Details）
GENPower™ NLS-Cas12a Nuclease is a recombinant Lachnospiraceae bacterium Cas12 protein purified from Escherichia coli for CRISPR-based genome editing. The introduction of nuclear localization signals (NLS) can help Cas12 enter the nucleus, increasing the chance of genomic DNA cleavage.
应用说明（Application）
Developed as a variety of genome editing tools, such as transcription regulation, gene recombination, and base editing.
浓度（Concentration）
10 μg/μL
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
无菌（Sterility）
Negative
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 20 mM NaAC, 500 mM NaCl, pH6.0 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
This product is stable after storage at:
- The product MUST be stored at -20°C or lower upon receipt;
- -20°C for 3 months under sterile conditions.
电泳（SDS-PAGE）
The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of GENPower™ NLS-Cas12a Nuclease (Cat. No. CAA-L5140) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.
Report
活性（Bioactivity）
Measured by its ability to cleave a targeted DNA substrate. Cas12a achieves >90% substrate cleavage (QC tested).
