膜杰作
Star Staining
描述（Description）
The Human IL-5 R alpha/CD131 (Luc) HEK293 Reporter Cell was engineered to not only express STAT5 signaling response element, but also express the receptors full length human IL-5 R alpha (Gene ID: 3568) and CD131 (Gene ID: 1439). When stimulated with human IL-5 protein, the IL-5/ IL-5 R alpha interaction drives STAT5-mediated luminescence. Inhibition of IL-5 binding to IL-5 R alpha by either anti-IL-5 or anti-IL-5 R alpha antibodies results in a decrease in luminescence.
应用说明（Application）
Screen for anti-human IL-5 or anti-human IL-5 R alpha neutralizing antibody.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
NA
培养基（Culture Medium）
DMEM medium + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Co-expression analysis of human IL-5 R alpha and CD131 on Human IL-5 R alpha/CD131 (Luc) HEK293 Reporter Cell by FACS.
Cell surface staining was performed on Human IL-5 R alpha/CD131 (Luc) HEK293 Reporter Cell or negative control cell using PE-labeled anti-IL-5 R alpha antibody and APC-labeled anti-CD131 antibody.
Protocol
Application
Inhibition of human IL-5 protein-induced reporter activity by anti-human IL-5 neutralizing antibody.
This reporter cell was incubated with serial dilutions of antibodies in the presence of human IL-5 protein (Cat. No. IL5-H52H3) with a final concentration of 0.004 μg/mL. The EC50 of anti-human IL-5 neutralizing antibody is approximately 0.22 μg/mL.
Protocol
Signaling Bioassay
Response to human IL-5 protein (RLU).
The Human IL-5 R alpha/CD131 (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-5 protein (Cat. No. IL5-H52H3). The EC50 was approximately 0.0045 μg/mL.
Protocol
Response to human IL-5 protein (Fold).
The Human IL-5 R alpha/CD131 (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-5 protein (Cat. No. IL5-H52H3). The max induction fold was approximately 23.
Protocol
Passage Stability
Passage stability analysis by Signaling Bioassay.
The continuously growing Human IL-5 R alpha/CD131 (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-5 protein. Human IL-5 protein stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 8-23.
Protocol
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背景（Background）
Interleukin-5 (IL-5) is a growth factor and chemoattractant for eosinophils and is considered to play a key role in allergic rhinitis, eosinophilic esophagitis and idiopathic hypereosinophilic syndrome. As the major cytokine in eosinophil development, IL-5 induces eosinophil migration, activation, and survival. Additionally, IL-5 has also been implicated in a range of conditions, such as asthma, cancer, and parasitic infections.
Permits & Restrictions
This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
