膜杰作
Star Staining
分子别名（Synonym）
Ephrin type-A receptor 10
表达区间及表达系统（Source）
Human EphA10, His Tag (EP0-H52H3) is expressed from human 293 cells (HEK293). It contains AA Pro 27 - Pro 564 (Accession # Q5JZY3-1).
Predicted N-terminus: Pro 27
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 60.2 kDa. The protein migrates as 65-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human EphA10, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Human EphA10, His Tag (Cat. No. EP0-H52H3) at 5 μg/mL (100 μL/well) can bind Human Ephrin-A3, Fc Tag (Cat. No. EA3-H5258) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Human Ephrin-A3, Fc Tag (Cat. No. EA3-H5258) at 5 μg/mL (100 μL/well) can bind Human EphA10, His Tag (Cat. No. EP0-H52H3) with a linear range of 0.005-0.313 μg/mL (Routinely tested).
Protocol
背景（Background）
At present, the proportion of breast cancer, cervical cancer and ovarian cancer in the occurrence of tumors that cause women's death has remained high. EPHA10 belongs to the EPH family, which is the largest known tyrosine kinase family, with 16 members. These members play a normal physiological function after interacting with their ligand Ephrin, and have been studied in sugar metabolism, new tissue construction, angiogenesis and other aspects. EPHA10 gene is located in 1p34 and mainly expressed in testis.
