TNF-alpha [Biotinylated] : TNFR1 Inhibitor Screening ELISA Kit

背景（Background）

Tumor Necrosis Factor (TNF), also known as TNFα, is a kind of cytokine that can kill tumor cells directly and has no obvious cytotoxicity to normal cells. TNF is a cell signaling protein involved in systemic inflammation, and it is one of the cytokines that constitute the acute phase response. It is produced primarily by activated macrophages, but most other immune cells, such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons in the nervous system can also produce it.

应用说明（Application）

This kit is developed for screening for inhibitors of human TNF-alpha binding to human TNFR1.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

Unopened kit should be stored at 2°C -8°C upon receiving. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

原理（Assay Principles）

This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new TNF-alpha pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human TNF-alpha to immobilized human TNFR1 in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated TNF-alpha protein, a human TNFR1 protein, an anti-TNF-alpha neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.

Your experiment will include 4 simple steps:

a) Coat the plate with human TNFR1 .

b) Add your molecule of interest to the tests.

c) Add human TNF-alpha-Biotin to bind the coated TNFR1.

d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

Finally, the half maximal inhibitory concentration (IC50) of your compound to TNF-alpha: TNFR1 binding will be determined by comparing OD readings among different experimental groups.