Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Assay Pair

组分（Materials Provided）

背景（Background）

Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. This ELISA assay pair contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human TNF-α.

应用说明（Application）

The pair is developed for quantitative detection of TNF-α in human serum and cell culture supernates.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

Unopened kit should be stored at 2°C -8°C upon receiving.

Find the expiration date on the outside packaging and do not use reagents past their expiration date.

The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

原理（Assay Principles）

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Human TNF-α. The kit consists of Human TNF-α Capture Antibody and Human TNF-α Standard and Human TNF-α Detection Antibody and Streptavidin-HRP.

Your experiment will include 5 simple steps:

a) Coat the plate with Human TNF-α Capture Antibody.

b) Add your molecule of interest to the tests.

c) Add Human TNF-α Detection Antibody.

d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis.