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ClinMax™ Human Interferon-γ (IFN-γ) ELISA Kit

For research use only.

产品概述(Product Details)

ClinMax™ Human Interferon-γ(IFN-γ) ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IFN-gamma that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:

  1. Standards to calibrate with NIBSC/WHO standards for comparable results.
  2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

应用说明(Application)

The kit is developed for quantitative detection of natural and recombinant human IFN-gamma in serum, plasma and cell culture supernatants.

It is suitable for potency assay of CAR-T/NK cell therapy.

It is for research use only.

流程图(Workflow)

IFN-gamma Workflow

关键信息(Key Features)

AnalyteIFN-γ
Assay TypeSandwich-ELISA
ReactivityHuman
Sensitivity5.309 pg/mL
Range19.5 pg/mL-1250 pg/mL
Assay Time2 hr 50 min
Sample TypeCell Culture Supernatants, Plasma, Serum.
Sample volume100 μL
Format96-wells plate breakable into 12 x 8 wells strips

Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

存储(Storage)

Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

 

典型数据-Typical Data Please refer to DS document for the assay protocol.

IFN-gamma TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of IFN-γ is less than 5.309 pg/mL.

 

验证(Validation)

样本值(Sample Values)

Cell Culture Supernatant – Human peripheral blood mononuclear cells (PBMCs) were cultured and stimulated using anti-CD3&CD28 magnetic beads and several concentrations of lipopolysaccharide (LPS) then detected IFN-γ secretion amount on Day 3 (72 hours).

IFN-gamma SAMPLE VALUES

稀释线性(Dilution Linearity)

High concentrations of human IFN-γ were added to different diluted substrates for gradient dilution to evaluate the linearity of the assay.

IFN-gamma DILUTION LINEARITY

批内差异(Intra-Assay Statistics)

20 replicates of each of 3 samples containing different IFN-γ concentrations were tested in one assay. Acceptable criteria: CV<10%.

IFN-gamma INTRA-ASSAY STATISTICS

批间差异(Inter-Assay Statistics)

3 samples containing different concentrations of IFN-γ were tested in independent assays. Acceptable criteria: CV<10%.

IFN-gamma INTER-ASSAY STATISTICS

回收率(Recovery)

IFN-γ was spiked into 3 human serum samples, and then analyzed. The average recovery of IFN-γ for serum samples is 93.5%, range is 85.2% - 105.2%.

IFN-gamma RECOVERY

 

组分(Materials Provided)

IDComponentsSize
CRS017-C01Pre-coated Anti-IFN-γ Antibody Microplate1 plate
CRS017-C02Human IFN-γ Standard30 μg
CRS017-C03Biotin-Anti-IFN-γ Antibody25 μg
CRS017-C04Streptavidin-HRP50 μL
CRS017-C0510xWashing Buffer 50 mL
CRS017-C062xDilution Buffer50 mL
CRS017-C07Substrate Solution12 mL
CRS017-C08Stop Solution7 mL

 

前沿进展

 
 
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IFN-gamma靶点信息
英文全称:Interferon gamma
中文全称:干扰素-γ
种类:Homo sapiens
上市药物数量:3详情
临床药物数量:9详情
最高研发阶段:批准上市
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