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ClinMax™ Human GM-CSF ELISA Kit

For research use only.

产品概述(Product Details)

ClinMax™ Human GM-CSF ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human GM-CSF that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:

  1. Standards to calibrate with NIBSC/WHO standards for comparable results.
  2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

应用说明(Application)

The kit is developed for quantitative detection of natural and recombinant human GM-CSF in serum, plasma and cell culture supernatants.

It is for research use only.

流程图(Workflow)

GM-CSF Workflow

关键信息(Key Features)

AnalyteGM-CSF
Assay TypeSandwich-ELISA
ReactivityHuman
Sensitivity10 pg/mL
Range12.5 pg/mL-1600 pg/mL
Assay Time1.75 hr
Sample TypeCell Culture Supernatants, Plasma, Serum.
Sample volume50 uL
Format96-wells plate breakable into 12 x 8 wells strips

Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

存储(Storage)

Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

 

典型数据-Typical Data Please refer to DS document for the assay protocol.

GM-CSF TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of GM-CSF is less than 10.0 pg/mL.

 

验证(Validation)

批内差异(Intra-Assay Statistics)

Ten replicates of each of 3 samples containing different GM-CSF concentrations were tested in one assay. Acceptable criteria: CV<10%.

GM-CSF INTRA-ASSAY STATISTICS

批间差异(Inter-Assay Statistics)

Three samples containing different concentrations of GM-CSF were tested in independent assays. Acceptable criteria: CV<15%.

GM-CSF INTER-ASSAY STATISTICS

回收率(Recovery)

Recombinant GM-CSF was spiked into 5 human serum samples, and then analyzed. The average recovery of GM-CSF for serum samples is 102.82%.

GM-CSF RECOVERY

 

组分(Materials Provided)

IDComponentsSize
CRB007-C01Pre-coated Anti-GM-CSF Antibody Microplate1 plate
CRB007-C02Human GM-CSF Standard32 μg×2
CRB007-C03Biotin-Anti-GM-CSF Antibody Con. Solution100 μL
CRB007-C04Biotin-Antibody Dilution Buffer8 mL
CRB007-C05Streptavidin-HRP Con. Solution500μL
CRB007-C06Streptavidin-HRP Dilution Buffer15 mL
CRB007-C0720× Washing Buffer50 mL
CRB007-C08Sample Dilution Buffer15 mL×2
CRB007-C09Substrate Solution12 mL
CRB007-C10Stop Solution6 mL

 

前沿进展

 
 
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010-53681107(北京)
021-50850665(上海)
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GM-CSF靶点信息
英文全称:Macrophage colony stimulating factor 2
中文全称:巨噬细胞集落刺激因子2
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:11详情
最高研发阶段:申请上市
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