膜杰作
Star Staining
抗体来源（Source）
Biotinylated Anti-His Tag Antibody(AY63), Fab is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
克隆号（Clone）
AY63
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
偶联（Conjugate）
Biotin
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Tag
免疫原（Immunogen）
A protein peptide with his tag.
特异性（Specificity）
This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.1-100 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>98% as determined by SEC-MALS.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70 °C for 3 months after reconstitution;
电泳（SDS-PAGE）
Biotinylated Anti-His Tag Antibody(AY63), Fab on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Anti-His Tag Antibody(AY63), Fab (Cat. No. HIS-BVM539) is more than 98% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (BQ.1/Omicron) (Cat. No. NUN-C52Hx) at 1μg/mL (100μL/well) can bind Biotinylated Anti-His Tag Antibody(AY63), Fab (Cat. No. HIS-BVM539) with a linear range of 0.2-100ng/mL (QC tested).
Protocol
背景（Background）
His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.
