膜杰作
Star Staining
产品参数（Product Specifications）
| Assay Type | Indirect-ELISA |
| Analyte | Anti-CD28 Antibody |
| Format | 96T(8×12 strips) |
| Reactivity | / |
| Regulatory Status | RUO |
| Sensitivity | ≤0.078ng/mL |
| Standard Curve Range | 0.078 ng/mL-2.5 ng/mL |
| Assay Time | 2 hr 20 min |
| Suitable Sample Type | For the detection of anti-CD28 antibody in Bioprocess manufacturing applications |
| Sample volume | 100 uL |
产品概述（Product Overview）
T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
2-8℃
组分（Materials Provided）
| ID | Components | Size |
| CRS014-C01 | Pre-coated Human CD28 Microplate | 1 plate(8×12 strips) |
| CRS014-C02 | Anti-CD28 Antibody Standard | 20 μg |
| CRS014-C03 | HRP-Goat anti-Mouse IgG | 10 μg |
| CRS014-C04 | 10xWashing Buffer | 50 mL |
| CRS014-C05 | 2xDilution Buffer | 50 mL |
| CRS014-C06 | Substrate Solution | 12 mL |
| CRS014-C07 | Stop Solution | 7 mL |
典型数据-Typical Data Please refer to DS document for the assay protocol.
Detection of Monoclonal Anti-CD28 Antibody titer by indirect-ELISA Assay.
Immobilized Human CD28 can bind Anti-CD28 Antibody. Detection was performed using HRP-Goat anti-Mouse IgG with sensitivity of 0.16 ng/mL (QC tested).
