膜杰作
Star Staining
分子别名（Synonym）
NT-3 growth factor receptor,GP145-TrkC,Neurotrophic tyrosine kinase receptor type 3, TrkC tyrosine kinase
表达区间及表达系统（Source）
Human TrkC Protein, Fc Tag (TRC-H5256) is expressed from human 293 cells (HEK293). It contains AA Cys 32 - Thr 429 (Accession # Q16288-1).
Predicted N-terminus: Cys 32
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 71.2 kDa. The protein migrates as 95-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human TrkC Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human TrkC Protein, Fc Tag (Cat. No. TRC-H5256) is more than 90% and the molecular weight of this protein is around 185-215 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human NT-3 / Neurotrophin-3 Protein at 2 μg/mL (100 μL/well) can bind Human TrkC Protein, Fc Tag (Cat. No. TRC-H5256) with a linear range of 0.3-5 ng/mL (QC tested).
Protocol
背景（Background）
Tropomyosin receptor kinase C (TrkC), also known as NT-3 growth factor receptor, neurotrophic tyrosine kinase receptor type 3, or TrkC tyrosine kinase is a protein that in humans is encoded by the NTRK3 gene. It belongs to the tropomyosin-related kinase (Trk) family of receptor tyrosine kinases (TrkA, TrkB, and TrkC).
TrkC is the high affinity catalytic receptor for the neurotrophin NT-3 (neurotrophin-3). As such, TrkC mediates the multiple effects of this neurotrophic factor, which includes neuronal differentiation and survival.
