膜杰作
Star Staining
分子别名（Synonym）
IFN-alpha 2a,IFNA2a,IFN-alpha 2 (alpha 2a),interferon alpha 2a
表达区间及表达系统（Source）
Human IFN-alpha 2a (R46K) Protein, Fc Tag (IFA-H5253) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 188 (R46K) (Accession # P01563 (R46K)).
Predicted N-terminus: Cys 24
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 45.7 kDa. The protein migrates as 48-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human IFN-alpha 2a (R46K) Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IFN-alpha 2a (R46K) Protein, Fc Tag (Cat. No. IFA-H5253) is more than 90% and the molecular weight of this protein is around 90-110 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IFNAR2, His Tag (Cat. No. IF2-H5224) at 5 μg/mL (100 μL/well) can bind Human IFN-alpha 2a (R46K) Protein, Fc Tag (Cat. No. IFA-H5253) with a linear range of 1-40 ng/mL (QC tested).
Protocol
背景（Background）
IFN-alpha is a cytokine that has an immunomodulatory function. It plays an important role not only in antiviral activity but also in several physiologic functions, such as activation of dendritic cells and accelerated expression of major histocompatibility complex I and II molecules that may cause increased antigen presentation. Variants of human leukocyte interferon alpha 2 (IFN-alpha 2a, alpha 2b, and alpha 2c) differ from each other by changes in their coding regions at nucleotide positions 137 and 170. IFN-alpha 2b (IFNα-2b) is an important cytokine and used for antiviral and anticancer treatment.
