登录 | 注册    关注公众号  
微信公众号
搜索
 >  Antibody>IgG control >DNP-BLM486

Biotinylated Mouse IgG2b Antibody Isotype Control (MALS verified)

抗体来源(Source)

Biotinylated Mouse IgG2b Antibody Isotype Control is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.

种属(Species)

Mouse

亚型(Isotype)

Mouse IgG2b | Mouse kappa

偶联(Conjugate)

Biotin

抗体类型(Antibody Type)

Recombinant Monoclonal

种属反应性(Reactivity)

Mouse

特异性(Specificity)

Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.

应用(Application)

ApplicationRecommended Usage
ELISA0.3-150 ng/mL 

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

纯化(Purification)

Protein A purified/ Protein G purified

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IgG control SDS-PAGE

Biotinylated Mouse IgG2b Antibody Isotype Control on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

IgG control SEC-MALS

The purity of Biotinylated Mouse IgG2b Antibody Isotype Control (Cat. No. DNP-BLM486) is more than 95% and the molecular weight of this protein is around 145-165 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

IgG control ELISA

Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse IgG2b Antibody Isotype Control (Cat. No. DNP-BLM486) with a linear range of 0.3-10 ng/mL (QC tested).

Protocol

 

背景(Background)

A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定