膜杰作
Star Staining
分子别名（Synonym）
FSTL1,FRP,FSL1
表达区间及表达系统（Source）
Biotinylated Human Follistatin-like 1 Protein, His,Avitag (FS1-H82E3) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Ile 308 (Accession # AAH00055).
Predicted N-terminus: Glu 21
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 35 kDa.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human Follistatin-like 1 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Human Follistatin-like 1 Protein, His,Avitag (Cat. No. FS1-H82E3) is more than 85% and the molecular weight of this protein is around 45-55 kDa verified by SEC-MALS.
Report
背景（Background）
Follistatin-related protein 1 (FSTL1) is also known as Follistatin-like protein 1 (FRP). FSTL1 is a secreted protein that contains two EF-hand domains, one follistatin-like domain, one Kazal-like domain and one VWFC domain. FSTL1 is overexpressed in synovial tissues from rheumatoid arthritis. Follistatin-like protein 1 / FSTL1 may modulate the action of some growth factors on cell proliferation and differentiation. FSTL1 binds heparin.
