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Biotinylated Human CDCP1 Protein, His,Avitag™ (MALS verified)

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分子别名(Synonym)

CUB domain-containing protein 1,CDCP1,CD318,Membrane glycoprotein gp140,Subtractive immunization M plus HEp3-associated 135 kDa protein,SIMA135,Transmembrane and associated with src kinases,TRASK,UNQ2486,PRO5773

表达区间及表达系统(Source)

Biotinylated Human CDCP1 Protein, His,Avitag (CD1-H82E4) is expressed from human 293 cells (HEK293). It contains AA Phe 30 - Thr 667 (Accession # Q9H5V8-1).

Predicted N-terminus: Phe 30

Request for sequence

蛋白结构(Molecular Characterization)

CDCP1 Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 75.7 kDa. The protein migrates as 90-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

CDCP1 SDS-PAGE

Biotinylated Human CDCP1 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

CDCP1 SEC-MALS

The purity of Biotinylated Human CDCP1 Protein, His,Avitag (Cat. No. CD1-H82E4) is more than 85% and the molecular weight of this protein is around 90-110 kDa verified by SEC-MALS.

Report

 

背景(Background)

CUB domain-containing protein 1, also know as CDCP1. CDCP1 is a cell surface glycoprotein that has been recognized both as a tumour marker and as a potential target to disrupt progression of cancer. CDCP1 has been shown to modulate cellsubstratum adhesion and motility in colon cancer cell lines.

 

前沿进展

 
 
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CDCP1靶点信息
英文全称:CUB domain-containing protein 1
中文全称:含CUB域蛋白1
种类:
上市药物数量:0详情
临床药物数量:0详情
最高研发阶段:临床前
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