膜杰作
Star Staining
抗体来源（Source）
HRP conjugated Anti-His Antibody (AY63), mAb is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
克隆号（Clone）
AY63
亚型（Isotype）
Human IgG1 | Human Kappa
偶联（Conjugate）
HRP-Conjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Tag
免疫原（Immunogen）
A protein peptide with his tag.
特异性（Specificity）
This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
应用（Application）
| Application | Recommended Usage |
| Western Blot | 0.2-2 ug/mL |
| ELISA | 1:3000-1:10000 |
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70 °C for 6 months after reconstitution.
- 2-8 °C for 2-3 weeks under sterile conditions after reconstitution.
活性（Bioactivity）-ELISA
Immobilized Canine TNF-alpha, His Tag (Cat. No. TNA-C52H3) at 1 μg/mL (100 μL/well) can bind HRP conjugated Anti-His Antibody (AY63), mAb (Cat. No. HIS-PLM535) with a linear range of 0.2-13 ng/mL (QC tested).
Protocol
Western Blot验证
Detection of HRP conjugated Anti-His Tag Antibody (AY63), mAb by Western Blot. HRP conjugated Anti-His Antibody (AY63), mAb at 1/10000 dilution + Human LAG-3 / CD223 Protein, His Tag at 75-250ng.
Predicted band size: 60 kDa 12% Bis-Tris Protein Gel.
背景（Background）
His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.
