膜杰作
Star Staining
& Cat.No. | Items | Details |
| Detection Method | Chemiluminescence |
| Product Type | Magnetic Beads (Protein L) |
| Quantity Size | 10mg / 100mg |
| Physical Appearance | lyophilized powder mixture |
| Particle size | 2.8µm |
| Beads Surface | Hydrophilic |
| Amount of Coupled Protein | About 474 pmol (20 μg) Protein L/mg Beads |
| Binding Capacity | 15-25ug Human antibody /mg beads |
| Emission Wavelength | Measured relative light units (RLU) at 430 nm |
背景（Background）
The Protein L-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly pure form of Protein L. The beads can be used to capture the antibodies in Chemiluminescence procedures. The Protein L is a 45 kDa surface protein, and can bind almost all antibody types, including IgG, IgM, IgA, IgE and IgD. It binds to antibodies with appropriate Kappa light chains such as VkI, VkIII and VkIV, whereas no binding occurred with antibodies of the VkII or with any Lambda light chains. The Protein L-coupled Magnetic Beads is easy to capture the most antibodies, and the bounded antibody have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.
应用说明（Application）
The Protein L-coupled Magnetic Beads is used to capture the most antibodies, it can combination with Acridinium ester markers in chemiluminescence technology. The Acridinium ester markers such as Streptavidin-Acridinium ester can capture the biotinylated proteins or molecules, this allows detection of Fc receptors and antibody binding.
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
Upon receipt, please store the Beads at -20℃. The shelf life is 1 year at -20 ℃.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
活性（Bioactivity）-MPCLIA
Immobilized 0.04 μg /Test of Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) to the Streptavidin-Acridine ester (Cat. No. STN-NA114, 0.02 μg /Test), incubated with 100 μL /Test of Trastuzumab at increasing concentration coupled to Protein L-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A008) (10 μg beads/Test). Detection was performed with sensitivity of 0.98 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
Protocol
The MPCLIA assay shows that Protein L-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A008) is stable at 37°C for 10 days.
The MPCLIA assay shows that Protein L-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A008) is stable after freezing and thawing 3 times.
The MPCLIA assay shows that Protein L-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A008) is stable at 37°C for 10 days.
