膜杰作
Star Staining
分子别名（Synonym）
Spike,S protein,Spike glycoprotein,S glycoprotein
表达区间及表达系统（Source）
Biotinylated MERS Spike Trimer Protein, His,Avitag (SPN-M82E3) is expressed from human 293 cells (HEK293). It contains AA Tyr 18 - Trp 1295 (Accession # K9N5Q8-1 (R748A, R751A, V1060P, L1061P, K1284Q)).
Predicted N-terminus: Tyr 18
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 147.6 kDa. The protein migrates as 180-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated MERS Spike Trimer Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated MERS Spike Trimer Protein, His,Avitag (Cat. No. SPN-M82E3) is more than 90% and the molecular weight of this protein is around 550-600 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human DPPIV, Fc Tag (Cat. No. DP4-H5266) at 5 μg/mL (100 μL/well) can bind Biotinylated MERS Spike Trimer Protein, His,Avitag (Cat. No. SPN-M82E3) with a linear range of 1-63 ng/mL (QC tested).
Protocol
Immobilized Biotinylated MERS Spike Trimer Protein, His,Avitag (Cat. No. SPN-M82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human DPPIV, Fc Tag (Cat. No. DP4-H5266) with a linear range of 0.004-0.25 μg/mL (Routinely tested).
Protocol
背景（Background）
The MERS or Middle East Respiratory Syndrome Coronavirus, is a member of the coronavirus family and is known to cause severe respiratory illness in humans. The MERS protein is a type I transmembrane protein that plays a vital role in the virus's ability to infect host cells. It is composed of three main domains: the extracellular domain, the transmembrane domain, and the cytoplasmic domain. The extracellular domain, further divided into S1 and S2 subunits, is responsible for receptor recognition and binding. The S1 subunit contains the Receptor Binding Domain (RBD), which is crucial for the virus's attachment to and entry into host cells. The RBD specifically interacts with the host cell receptor, facilitating the initial step of infection. The S2 subunit, on the other hand, is responsible for membrane fusion. It contains a fusion peptide that inserts into the host cell membrane, initiating the fusion process. This fusion allows the virus's genetic material to be released into the host cell, initiating the infection process.
