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 >  Protein>ALPI >ALI-H52H3

Human ALPI Protein, His Tag (MALS verified)

分子别名(Synonym)

IAP

表达区间及表达系统(Source)

Human ALPI Protein, His Tag (ALI-H52H3) is expressed from human 293 cells (HEK293). It contains AA Val 20 - Asp 503 (Accession # P09923-1).

Predicted N-terminus: Val 20

Request for sequence

蛋白结构(Molecular Characterization)

ALPI Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 54.3 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Supplied as 0.2 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 3 months under sterile conditions.
 

电泳(SDS-PAGE)

ALPI SDS-PAGE

Human ALPI Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

ALPI SEC-MALS

The purity of Human ALPI Protein, His Tag (Cat. No. ALI-H52H3) is more than 90% and the molecular weight of this protein is around 100-130 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

ALPI ELISA

Immobilized Human ALPI Protein, His Tag (Cat. No. ALI-H52H3) at 1 μg/mL (100 μL/well) can bind various dilution ratio of Anti-ALPI Polyclonal Antibody (QC tested).

Protocol

 

背景(Background)

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut.

 

前沿进展

 
 
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ALPI靶点信息
英文全称:Intestinal alkaline phosphatase
中文全称:肠碱性磷酸酶
种类:
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床二期
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