膜杰作
Star Staining
描述（Description）
The Human IL-21 R/CD132 (Luc) HEK293 Reporter Cell was engineered to not only express STAT3 signaling response element, but also express the receptors full length human IL-21 R (Gene ID:50615) and CD132 (Gene ID:3561). When stimulated with human IL-21 protein, the IL-21/ IL-21 R interaction drives STAT3-mediated luminescence. Inhibition of IL-21 binding to IL-21 R by either anti-IL-21 or anti-IL-21 R antibodies results in a decrease in luminescence.
应用说明（Application）
• Screen for anti-human IL-21 or anti-human IL-21 R neutralizing antibody.
生长特性（Growth Properties）
Adherent
培养基（Culture Medium）
DMEM medium + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Co-expression analysis of human IL-21 R and CD132 on Human IL-21 R/CD132 (Luc) HEK293 Reporter Cell by FACS.
Cell surface staining was performed on Human IL-21 R/CD132 (Luc) HEK293 Reporter Cell or negative control cell using BV421-labeled anti-IL-21 R antibody and PE-labeled anti-CD132 antibody.
Protocol
Application
Inhibition of human IL-21 protein-induced reporter activity by anti-human IL-21 neutralizing antibody.
This reporter cell was incubated with serial dilutions of antibodies in the presence of human IL-21 protein (Cat. No. IL1-H5213) with a final concentration of 0.01 μg/mL. The EC50 of anti-human IL-21 neutralizing antibody is approximately 0.1545 μg/mL.
Protocol
Signaling Bioassay
Response to human IL-21 protein (RLU).
The Human IL-21 R /CD132 (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-21 protein (Cat. No. IL1-H5213). The EC50 was approximately 0.0068 μg/mL.
Protocol
Response to human IL-21 protein (Fold).
The Human IL-21 R /CD132 (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-21 protein (Cat. No. IL1-H5213). The max induction fold was approximately 46.1.
Protocol
Passage Stability
Passage stability analysis by Signaling Bioassay.
The continuously growing Human IL-21 R/CD132 (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-21 protein. Human IL-21 protein stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 7-21.
Protocol
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背景（Background）
Interleukin-21 receptor (IL21R) is also known as CD antigen CD360, Novel interleukin receptor (NILR), is a receptor for interleukin-21 and belongs to the type I cytokine receptor family or type 4 subfamily. IL21R contains two fibronectin type-III domains. IL21R is selectively expressed in lymphoid tissues and most highly expressed in thymus and spleen. IL21R transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells.
Permits & Restrictions
This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
