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HEK293/Human Glypican-3 (GPC3) Stable Cell Line

For research use only.

描述(Description)

The HEK293/Human Glypican-3 (GPC3) Stable Cell Line was engineered to express the receptor full length human GPC3 (Gene ID: 2719), used to mimic cancer target cells. Surface expression of Human GPC3 was confirmed by flow cytometry.

应用说明(Application)

• Useful for cell-based GPC3 binding assay

生长特性(Growth Properties)

Adherent

筛选标记(Selection Marker)

Puromycin (2 μg/mL)

培养基(Culture Medium)

DMEM medium + 10% FBS

冻存液(Freeze Medium)

Serum-free cell cryopreservation medium

装量(Quantity)

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

存储(Storage)

Frozen in liquid nitrogen.

支原体检测(Mycoplasma Testing)

Negative

无菌检测(Sterility Testing)

Negative

使用说明(Instructions for Use)

See data sheet for detailed culturing and assay protocol.

 

Receptor Assay

Glypican 3 FACS

Expression analysis of human GPC3 on HEK293/Human Glypican-3 (GPC3) Stable Cell Line by FACS.
Cell surface staining was performed on HEK293/Human Glypican-3 (GPC3) Stable Cell Line or negative control cell using PE-labeled anti-human GPC3 antibody.

Protocol

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背景(Background)

Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

 

Permits & Restrictions

This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.

 

前沿进展

 
 
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Glypican 3靶点信息
英文全称:Glypican-3
中文全称:磷脂酰肌醇聚糖3
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:36详情
最高研发阶段:临床二期
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