膜杰作
Star Staining
描述（Description）
The Human IL-31 RA/OSMR (Luc) HEK293 Reporter Cell was engineered to express STAT3 signaling response element, but also express the receptors full length human IL-31 RA (Gene ID: 133396) and OSMR (Gene ID: 9180). When stimulated with human IL-31 protein, the IL-31/IL-31 RA interaction drives STAT3-mediated luminescence. Neutralization of biological effect of human IL-31 protein by corresponding antibody results in a decrease in luminescence.
应用说明（Application）
• Screen for neutralizing antibodies blocking the stimulation of human IL-31 protein.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL) + Hygromycin (40 μg/mL) + Zeocin (20μg/mL)
培养基（Culture Medium）
DMEM medium + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Co-expression analysis of human IL-31 RA and OSMR on Human IL-31 RA/OSMR (Luc) HEK293 Reporter Cell by FACS.
Cell surface staining was performed on Human IL-31 RA/OSMR (Luc) HEK293 Reporter Cell or negative control cell using PE-labeled anti-human IL-31 RA antibody and APC-labeled anti-human OSMR antibody.
Protocol
Application
Inhibition of human IL-31 protein-induced reporter activity by anti-human IL-31 RA neutralizing antibody.
This reporter cell was incubated with serial dilutions of antibodies in the presence of human IL-31 protein (Cat. No. IL1-H5247) with a final concentration of 0.001 μg/mL. The EC50 of anti-human IL-31 RA neutralizing antibody is approximately 0.609 μg/mL.
Protocol
Inhibition of human IL-31 protein-induced reporter activity by anti-human OSMR neutralizing antibody.
This reporter cell was incubated with serial dilutions of antibodies in the presence of human IL-31 protein (Cat. No. IL1-H5247) with a final concentration of 0.001 μg/mL. The EC50 of anti-human OSMR neutralizing antibody is approximately 0.727 μg/mL.
Protocol
Signaling Bioassay
Response to human IL-31 protein (RLU).
The Human IL-31 RA/OSMR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-31 protein (Cat. No. IL1-H5247). The EC50 was approximately 0.0102 μg/mL.
Protocol
Response to human IL-31 protein (FOLD).
The Human IL-31 RA/OSMR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-31 protein (Cat. No. IL1-H5247). The max induction fold was approximately 328.
Protocol
Passage Stability
Passage stability analysis by Signaling Bioassay.
The continuously growing Human IL-31 RA/OSMR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-31 protein. Human IL-31 protein stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 9-20.
Protocol
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背景（Background）
Interleukin-31 receptor subunit alpha is a protein that in humans is encoded by the IL31RA gene, also known as IL-31 receptor subunit alpha, IL-31RA, GLM-R, Gp130-like receptor, CRL3, GPL. Oncostatin M receptor (OSMR) and IL31RA form the heterodimeric receptor through which IL31 signals. IL31RA is a strong activator of STAT3 and STAT5, whereas STAT1 is only marginally tyrosine-phosphorylated. dditionally, demonstrate Jak1 binding to GPL and its activation in heteromeric complexes with the OSMR beta but also in a homomeric receptor complex.
Permits & Restrictions
This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
