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 >  Antibody>Adalimumab >ADB-BY17c

Biotinylated Anti-Adalimumab Antibody (22E7A4A2) (recommended for PK/PD)

抗体来源(Source)

Biotinylated Anti-Adalimumab Antibody (22E7A4A2) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.

克隆号(Clone)

22E7A4A2

种属(Species)

Mouse

亚型(Isotype)

Mouse IgG1 | Mouse Kappa

抗体类型(Antibody Type)

Recombinant Monoclonal

种属反应性(Reactivity)

Human

免疫原(Immunogen)

Adalimumab.

特异性(Specificity)

Recognizes Adalimumab specifically.

应用(Application)

ApplicationRecommended Usage
ELISA0.4-12.5 ng/mL

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

纯化(Purification)

Protein A purified/ Protein G purified

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Adalimumab SDS-PAGE

Biotinylated Anti-Adalimumab Antibody (22E7A4A2) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Adalimumab SEC-MALS

The purity of Biotinylated Anti-Adalimumab Antibody (22E7A4A2) (Cat. No. ADB-BY17c) is more than 90% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Adalimumab ELISA

Immobilized Adalimumab at 1 μg/mL (100 μL/well) can bind Biotinylated Anti-Adalimumab Antibody (22E7A4A2) (Cat. No. ADB-BY17c) with a linear range of 0.4-3 ng/mL (QC tested).

Protocol

 

背景(Background)

Adalimumab is a human monoclonal antibody against TNF-alpha. It is produced by recombinant DNA technology using a mammalian cell expression system. It consists of 1330 amino acids and has a molecular weight of approximately 148 kilodaltons.

 

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