分子别名(Synonym)
LILRB2,ILT4,LIR2,MIR10,CD85d
表达区间及表达系统(Source)
Biotinylated Human LILRB2 Protein, His,Avitag (LI2-H82E7) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Val 461 (Accession # Q8N423-1).
Predicted N-terminus: Gln 22
Request for sequence
蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)
The protein has a calculated MW of 51.3 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Biotinylated Human LILRB2 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Human LILRB2 Protein, His,Avitag (Cat. No. LI2-H82E7) is more than 90% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Anti-LILRB2 Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human LILRB2 Protein, His,Avitag (Cat. No. LI2-H82E7) with a linear range of 0.3-20 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human LILRB2 Protein, His,Avitag (Cat. No. LI2-H82E7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-LILRB2 Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
背景(Background)
Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2) is also known as CD85 antigen-like family member D (CD85d), Immunoglobulin-like transcript 4 (ILT-4), Monocyte / macrophage immunoglobulin-like receptor 10 (MIR-10), which is a member of the the subfamily B class of LIR receptors. LILRB2 is receptor for class I MHC antigens. LILRB2 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB2 competes with CD8A for binding to class I MHC antigens. LILRB2 / CD85d inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.