膜杰作
Star Staining
分子别名（Synonym）
PERB11.2
表达区间及表达系统（Source）
Human MICB Protein, His Tag (MIB-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 23 - Thr 308 (Accession # NP_005922.2).
Predicted N-terminus: Ala 23
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 34.5 kDa. The protein migrates as 45-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human MICB Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human MICB Protein, His Tag (Cat. No. MIB-H52H3) is more than 85% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human NKG2D Protein, Fc Tag (Cat. No. NKD-H5265) at 5 μg/mL (100 μL/well) can bind Human MICB Protein, His Tag (Cat. No. MIB-H52H3) with a linear range of 0.039-0.625 μg/mL (QC tested).
Protocol
Immobilized Human NKG2D, Mouse IgG2a Fc Tag (Cat. No. NKD-H5259) at 5 μg/mL (100 μL/well) can bind Human MICB Protein, His Tag (Cat. No. MIB-H52H3) with a linear range of 0.039-0.313 μg/mL (Routinely tested).
Protocol
背景（Background）
Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
