膜杰作
Star Staining
抗体来源（Source）
Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (5F8E1) (XBB.1.5/Omicron Specific) is isolated from a Spike RBD infected Mouse and is recombinantly produced from Hybridoma.
克隆号（Clone）
5F8E1
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Hybridoma Monoclonal
种属反应性（Reactivity）
Virus
免疫原（Immunogen）
Recombinant SARS-CoV-2 Spike RBD Protein (XBB.1.5/Omicron) erived from human 293 cells.
特异性（Specificity）
This product is a specific antibody specifically reacts with SARS-CoV-2 Spike Trimer Protein, His Tag (XBB/Omicron) (Cat. No. SPN-C5248), SARS-CoV-2 Spike Trimer Protein, His Tag (XBB.1/Omicron) (Cat. No. SPN-C522t) and SARS-CoV-2 Spike Trimer Protein, His Tag (XBB.1.5/Omicron) (Cat. No. SPN-C524i). No cross-reactivity is detected with Spike Protein of WT (Cat. No. SPN-C52H9), Alpha (Cat. No. SPN-C52H6), Beta, Gamma (Cat. No. SPN-C52Hg), Delta (Cat. No. SPN-C52He), B.1.1.529/Omicron (Cat. No. SPN-C52Hz), BA.2/Omicron (Cat. No. SPN-C5223), BA.3/Omicron (Cat. No. SPN-C5225), BA.4/Omicron (Cat. No. SPN-C5229), BA.5/Omicron (Cat. No. SPN-C522e), BA.2.12.1/Omicron (Cat. No. SPN-C522d), BQ.1.1/Omicron (Cat. No. SPN-C522s), BA.2.75/Omicron (Cat. No. SPN-C522f), BA.4.6/Omicron (Cat. No. SPN-C522m), BF.7/Omicron (Cat. No. SPN-C522q).
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.4-200 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (5F8E1) (XBB.1.5/Omicron Specific) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized SARS-CoV-2 Spike Trimer Protein, His Tag (XBB/Omicron) (MALS verified) (Cat. No. SPN-C5248), SARS-CoV-2 Spike Trimer Protein, His Tag (XBB.1/Omicron) (MALS verified) (Cat. No. SPN-C522t) and SARS-CoV-2 Spike Trimer Protein, His Tag (XBB.1.5/Omicron) (MALS verified) (Cat. No. SPN-C524i) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (5F8E1) (XBB.1.5/Omicron Specific) (Cat. No. SPD-Y171) with a linear range of 0.098-6.25 ng/mL (QC tested). The antibody does not bind Spike Protein of WT (Cat. No. SPN-C52H9), Alpha (Cat. No. SPN-C52H6), Beta, Gamma (Cat. No. SPN-C52Hg), Delta (Cat. No. SPN-C52He), B.1.1.529/Omicron (Cat. No. SPN-C52Hz), BA.2/Omicron (Cat. No. SPN-C5223), BA.3/Omicron (Cat. No. SPN-C5225), BA.4/Omicron (Cat. No. SPN-C5229), BA.5/Omicron (Cat. No. SPN-C522e), BA.2.12.1/Omicron (Cat. No. SPN-C522d), BQ.1.1/Omicron (Cat. No. SPN-C522s), BA.2.75/Omicron (Cat. No. SPN-C522f), BA.4.6/Omicron (Cat. No. SPN-C522m), BF.7/Omicron (Cat. No. SPN-C522q) (QC tested).
Protocol
背景（Background）
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
