膜杰作
Star Staining
分子别名（Synonym）
MIC-A
表达区间及表达系统（Source）
Cynomolgus MICA Protein, His Tag (MIA-C52H5) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Trp 304 (Accession # A0A7N9D4J5).
Predicted N-terminus: Leu 25
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 33.9 kDa. The protein migrates as 48-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Cynomolgus MICA Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Cynomolgus MICA Protein, His Tag (Cat. No. MIA-C52H5) was greater than 85% as determined by SEC-HPLC.
活性（Bioactivity）-ELISA
Immobilized Human NKG2D, Mouse IgG2a Fc Tag (Cat. No. NKD-H5259) at 2 μg/mL (100 μL/well) can bind Cynomolgus MICA Protein, His Tag (Cat. No. MIA-C52H5) with a linear range of 0.02-0.313 μg/mL (QC tested).
Protocol
Immobilized Human NKG2D Protein, Fc Tag (Cat. No. NKD-H5265) at 5 μg/mL (100 μL/well) can bind Cynomolgus MICA Protein, His Tag (Cat. No. MIA-C52H5) with a linear range of 0.008-0.25 μg/mL (Routinely tested).
Protocol
Immobilized Cynomolgus MICA Protein, His Tag (Cat. No. MIA-C52H5) at 5 μg/mL (100 μL/well) can bind Human NKG2D Protein, Fc Tag (Cat. No. NKD-H5265) with a linear range of 0.039-1.25 μg/mL (Routinely tested).
Protocol
背景（Background）
MHC class I polypeptide-related sequence A (MICA) belongs to the MHC class I family and MIC subfamily. MICA contains one Ig-like C1-type (immunoglobulin-like) domain. Unlike classical MHC class I molecules, MICA does not form a heterodimer with beta-2-microglobulin. MICA acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. MICA is ligand for the KLRK1/NKG2D receptor. MICA bind to KLRK1 leads to cell lysis.
