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 >  Antibody>FMC63 >FM3-FY45G0

FITC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45), premium grade

抗体来源(Source)

FITC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45), premium grade is expressed from human HEK293 cells. It is the FITC labeled form of Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Cat. No. FM3-Y45P1).

It is designed for cell isolation and cell culture applications, and produced under animal-free manufacturing conditions. No animal derived components are used throughout the production process.

It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

亚型(Isotype)

Mouse IgG1/kappa

偶联(Conjugate)

FITC

特异性(Specificity)

Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.

内毒素(Endotoxin)

Less than 0.1 EU per test by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

蛋白标记度(Protein Ratio)

The FITC to protein molar ratio is 1-4.

无菌(Sterility)

Negative

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

This product is stable after storage at:

  1. -20°C to -70°C for 24 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution;
  3. 2-8°C for 12 months under sterile conditions after reconstitution.
 

活性(Bioactivity)-FACS

FMC63 FACS

2e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of FITC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45), premium grade (Cat. No. FM3-FY45G0) and Isotype control antibody respectively. FITC signal was used to evaluate the binding activity (QC tested).

Protocol

 

背景(Background)

FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.

 

前沿进展

 
 
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