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 >  Protein>Laminin 211 >LA8-H5263

Human Laminin 211 Protein, premium grade

产品描述(Product Details)

Laminin-α2 is a critical component of the laminin heterotrimer, which along with laminin-β1 or β2 and laminin-γ1 form the structural glycoproteins laminin-211 and laminin-221 in skeletal muscle. Laminin-211 is the most abundant laminin isoform in the basement membrane of adult skeletal muscle. Laminin-211 and -221 polymerize with each other and interact with nidogen and collagen-IV to form the muscle basal lamina. Laminin-211 and laminin-221 bind to muscle cell surface through the α7β1 integrin and α-dystroglycan of the dystrophin glycoprotein complex via their globular C-terminal domains.

产品参数(Key parameter)

Purity (SDS PAGE)> 90%
Mycoplasma TestNegative
Sterility TestNegative
Endotoxin Test< 0.1 EU per μg

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

活性(Bioactivity)-ELISA

Laminin 211 ELISA

Immobilized Human Laminin 211 Protein, premium grade (Cat. No. LA8-H5263) at 5 μg/mL (100 μL/well) can bind Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) with a linear range of 0.02-0.313 μg/mL (QC tested).

Protocol

 

前沿进展

 
 
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