膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-HA (A/Wisconsin/588/2019 (H1N1)) Antibody, Mouse IgG1 (6D1) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.
克隆号（Clone）
6D1
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Hybridoma Monoclonal
种属反应性（Reactivity）
Virus
免疫原（Immunogen）
Recombinant Influenza A [A/Wisconsin/588/2019 (H1N1)] HA derived from human 293 cells.
特异性（Specificity）
This product is a specific antibody specifically reacts with Hemagglutinin/HA (Influenza Virus).
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.1-100 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
交叉验证（Cross Verification）
This product can cross in Elisa with
Influenza A [Victoria/4897/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H8).
Influenza A [Wisconsin/67/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H7).
Influenza A [A/Victoria/2570/2019] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H6).
Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H14).
No cross-reactivity in ELISA with
Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
Influenza A [A/Darwin/9/2021 (H3N2)] HA Protein, His Tag (Cat. No.HA2-V52H6).
Influenza A [A/Hong Kong/483/97 (H5N1)] HA, His Tag (Cat. No. HA1-V5229).
Influenza A (Guangdong/18SF020(H5N6)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA6-V52H3).
Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (Cat. No. HA8-V52H3).
Influenza A (A/Shanghai/02/2013(H7N9)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA9-V52H3).
Influenza B [Austria/1359417/2021 (B/Victoria lineage)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H3).
Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag (Cat. No. HAE-V52H4).
Influenza A (Vietnam/1194/2004(H5N1)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H9).
Influenza A Virus (A/District of Columbia/27/2023) HA (H3N2) Protein, His Tag (Cat. No. H32-V52H5).
Influenza A Virus (A/Croatia/10136RV/2023) HA (H3N2) Protein, His Tag (Cat. No. H32-V52H4).
电泳（SDS-PAGE）
Monoclonal Anti-HA (A/Wisconsin/588/2019 (H1N1)) Antibody, Mouse IgG1 (6D1) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-HA (A/Wisconsin/588/2019 (H1N1)) Antibody, Mouse IgG1 (6D1) (Cat. No. HA1-Y159) is more than 95% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Influenza A [A/Wisconsin/588/2019 (H1N1)] Hemagglutinin (HA) Protein, His Tag (MALS verified) (Cat. No. HA1-V52H3) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-HA (A/Wisconsin/588/2019 (H1N1)) Antibody, Mouse IgG1 (6D1) (Cat. No. HA1-Y159) with a linear range of 0.049-3.125 ng/mL. (QC tested).
Protocol
背景（Background）
Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Hemagglutinin also plays a major role in the determination of host range restriction and virulence. As a class I viral fusion protein, hemagglutinin is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.
