膜杰作
Star Staining
分子别名（Synonym）
Gn protein,glycoprotein polyprotein,Phlebovirus_G1,Phlebovirus_G2
表达区间及表达系统（Source）
SFTS virus Gn Protein (HN6), His Tag (GNN-S52H4) is expressed from human 293 cells (HEK293). It contains AA Asp 20 - Lys 452 (Accession # F1BWU4).
Predicted N-terminus: Asp 20
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蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 49.2 kDa. The protein migrates as 55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
SFTS virus Gn Protein (HN6), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of SFTS virus Gn Protein (HN6), His Tag (Cat. No. GNN-S52H4) is more than 90% and the molecular weight of this protein is around 95-120 kDa verified by SEC-MALS.
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背景（Background）
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging viral hemorrhagic fever (VHF) endemic to China, South Korea, Japan, and Vietnam. Severe fever with thrombocytopenia syndrome (SFTS) is an infectious disease with a high fatality rate, caused by SFTS virus (SFTSV). To our knowledge, no efficient SFTSV vaccine exists.
