膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
宿主（Host Species）
Mouse
亚型（Isotype）
Human IgG1/kappa
特异性（Specificity）
This product is a specific antibody specifically reacts with His.
抗体类型（Antibody Type）
Recombinant Monoclonal
纯度（Purity）
>95% as determined by SDS-PAGE.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
偶联（Conjugate）
Acridine ester
蛋白标记度（Protein Ratio）
Passed as determined by binding MPCLIA.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH6.3 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (Cat. No. HIS-FM535) is more than 85% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-MPCLIA
Immobilized 0.025 μg /Test of SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) to the Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (Cat. No. HIS-FM535, 0.04 μg /Test), incubated with 100 μL /Test of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Mouse IgG1 (AM122) (Cat. No. SCT-M369) at increasing concentration coupled to Anti-Mouse IgG-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A003) (10 μg beads/Test). Detection was performed with sensitivity of 0.98 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
Protocol
The MPCLIA assay shows that Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (Cat. No. HIS-FM535) is stable at 37°C for 48 hours.
Protocol
The MPCLIA assay shows that Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (Cat. No. HIS-FM535) is stable after freezing and thawing 3 times.
Protocol
背景（Background）
His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.
