登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>B7-H4 >B74-H8222

Biotinylated Human B7-H4 Protein, His Tag, ultra sensitivity (primary amine labeling)

热销产品推荐:

利用生物素-链霉亲和素系统的高特异性和高灵敏度的结合特性,可将生物素化蛋白与链霉亲和素相关产品联合应用于各类实验,与非生物素体系相比,检测速度更快、结合能力更强、非特异背景更低。

分子别名(Synonym)

B7-H4,VTCN1,B7S1,B7h.5

表达区间及表达系统(Source)

MABSol® Biotinylated Human B7-H4, His Tag, primary amine labeling (B74-H8222) is expressed from human HEK293 cells. It contains AA Phe 29 - Ala 258 (Accession # NP_078902 ). It is the biotinylated form of Human B7-H4, His Tag (Cat. No. B74-H5222).

Predicted N-terminus: Phe 29

Request for sequence

蛋白结构(Molecular Characterization)

B7-H4 Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 26.4 kDa. The protein migrates as 43-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

B7-H4 SDS-PAGE

Biotinylated Human B7-H4, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

B7-H4 ELISA

Immobilized Anti-Human B7-H4 mAb at 2 μg/mL (100 μL/well) can bind Biotinylated Human B7-H4, His Tag, primary amine labeling (Cat. No. B74-H8222) with a linear range of 1-8 ng/mL (QC tested).

Protocol

 

背景(Background)

V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs). VTCN1 / B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.

 

前沿进展

 
 
货号/价格
产品推荐
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
B7-H4靶点信息
英文全称:V-set domain-containing T-cell activation inhibitor 1
中文全称:含V区域T细胞激活抑制因子1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:9详情
最高研发阶段:临床二期
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定