膜杰作
Star Staining
分子别名（Synonym）
HLA-A*1101 & B2M & KRAS (VVVGAGGVGK)
表达区间及表达系统（Source）
Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (HLS-HA2H8) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & VVVGAGGVGK peptide (Accession # Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & VVVGAGGVGK).
Predicted N-terminus: Gly 25 & Ile 21
Request for sequence
蛋白结构（Molecular Characterization）
Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRAS peptide.
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 49.4 kDa and 11.7 kDa. The protein migrates as 57-75 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
AF647
Excitation Wavelength: 640 nm
Emission Wavelength: 672 nm
标记（Labeling）
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF647 using standard chemical labeling method. The residual AF647 is removed by molecular sieve treatment during purification process.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Cat. No. HLS-HA2H8) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Cat. No. HLS-HA2H8) at 1 μg/mL (100 μL/well) can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Cat. No. HLS-HA2H8) captured on CM5 Chip via Anti-B2M antibody can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with an affinity constant of 0.117 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
The Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) oncogene plays a critical role in the initiation and maintenance of pancreatic tumors and its signaling network represents a major target for therapeutic intervention. The Alex Fluor 647 labeled Human HLA-A*1101 KRAS (VVVGAGGVGK) complex protein is a complex of HLA-A*1101 of the MHC Class I, B2M, and VVVGAGGVGK peptide of the KRAS.
