膜杰作
Star Staining
产品描述（Product Details）
| Assay Type | Sandwich-FCM |
| Analyte | IFN-γ |
| Format | 96T |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | 2.0 pg/mL |
| Standard Curve Range | 9.8 pg/mL-10000 pg/mL |
| Assay Time | 2 hr 30 min |
| Suitable Sample Type | For the quantitative determination of human IFN-γ in Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 30 μL |
背景（Background）
The biology and function of IFN-γ has been extensively reviewed in the literature. Cytometric Bead Array (CBA) assays provide a method of capturing a soluble analyte or set of analytes with beads of known size and fluorescence, making it possible to detect analytes using flow cytometry.
应用说明（Application）
Human IFN-γ Kit (Flow Cytometry Beads Assay) is a bead-based immunoassay capable of measuring human interferon-γ (IFN-γ) in serum, plasma, and cell culture supernatant samples.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
The unopened kit is stable for 18 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
| ID | Components | Size |
| CFA001-C01 | Calibrator | 20 μg |
| CFA001-C02 | IFN-γ Beads | 96 tests |
| CFA001-C03 | 2× Assay Buffer | 40 mL |
| CFA001-C04 | 10× Wash Buffer | 10 mL |
| CFA001-C05 | Detection antibody | 1 mL |
| CFA001-C06 | SA-PE | 40 μg |
| CFA001-C08 | 96-well Sealing film | 2 pieces |
| CFA001-C09 | APC Positive Control | 500 μL |
| CFA001-C10 | PE Positive Control | 500 μL |
原理（Assay Principles）
This assay kit is used to measure the concentrations of Human Interferon-γ (IFN-γ) by employing a standard sandwich-FCM format. The antibody encapsulated 1-plex beads, protein (IFN-γ) in specimens and biotin-conjugated detection antibody formed a sandwich complex as bead-analyte-detection antibody. SA-PE was added in and react with biotinylated detection antibody, the intensity of PE fluorescence, in proportion to the recombinant protein titer in specimens, was assessed by flow cytometry at wavelength of 575 nm approximately. The intensity of APC fluorescence was applied to classify bead populations, at wavelength of 670 nm approximately.
典型数据-Typical Data Please refer to DS document for the assay protocol.
For each experiment, each 96-well V-bottom plate needs to set the standard curve. The minimum detectable concentration of CFA001-C02 is less than 2.0 pg/mL.
背景（Background）：IFN-gamma
Interferon-gamma ,Interferon-γ
Interferon-gamma (IFN-γ/IFNG) is a dimerized soluble cytokine that is the only member of the type II class of interferon. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-γ belongs. IFN-gamma has been used in a wide variety of clinical indications. Interferon-gamma (IFNgamma) is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway. Interferon gamma has broader roles in activation of innate and adaptive immune responses to viruses and tumors, in part through upregulating transcription of genes involved in cell cycle regulation, apoptosis, and antigen processing/presentation. Despite this, rodent and human trophoblast cells show dampened responses to IFNG that reflect the resistance of these cells to IFNG-mediated activation of major histocompatibility complex (MHC) class II transplantation antigen expression.
